Molecular Characterization Of Endophytic Bacterial Isolates Of Angsana Leaves (Pterocarpus Indicus Willd) Producers Of Antibiotics Compounds

The increasing resistance of pathogenic bacteria to antimicrobials requires the discovery of new antibiotics. Angsana plant or Lansano (Pterocarpus indicus Willd) is one of the medicinal plants in West Sumatra. Angsana has the potential to have endophytic bacteria capable of producing antibiotic compounds like its host. The endophytic bacterial isolates of Angsana can inhibit the growth of S. aureus and E. coli bacteria. This study aims to determine the genetic characteristics of the endophytic bacteria of Angsana leaves that produce antibiotic compounds through phylogenetic tree analysis. The type of research is descriptive qualitative which was carried out from May to October 2019 at the Biotechnology Laboratory of UNP Padang. The process of amplification of the 16S rRNA gene from 3 isolates has succeeded in obtaining a single DNA band located between the 1494 bp, 1465 bp and 1163 bp DNA bands of the HindIII λ marker. The size of the PCR product for each isolate that had been amplified was approximately 1500 bp. The BLAST results showed that the bacterial isolates were identified as one genus, namely the genus Bacillus with the types Bacillus tequilensis and Bacillus subtilis. The endophytic bacterial isolate DB 1 was related to Bacillus tequilensis. Bacterial isolates DB2 and DA 3 were most closely related to Bacillus subtilis.